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以甜菜夜蛾(Spodoptera exigua)和模式植物拟南芥(Arabidopsis thanliana)作为研究体系,应用蛋白质双向凝胶电泳(Two-dimensional gel electrophoresis,2-DE)分析了在甜菜夜蛾取食诱导条件下拟南芥蛋白表达的差异,从蛋白质水平揭示昆虫取食诱导条件下植物的化学防御机制.结果发现,在昆虫取食诱导条件下,有28个蛋白发生显著变化,其中17个蛋白点上调表达,11个蛋白点下调表达.利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对差异蛋白进行了鉴定,结果发现转酮酶、S-腺苷甲硫氨酸合成酶、二氢硫辛酰胺脱氢酶和脂肪酸合成酶在植物诱导化学防御中具有重要的作用,其中脂肪酸合成酶与茉莉酸代谢通路相关.
Using Spodoptera exigua and Arabidopsis thanliana as the researching system, we analyzed the effect of the induced insecticidal activity of beet armyworm on two-dimensional gel electrophoresis (2-DE) Arabidopsis thaliana protein expression differences revealed from the level of protein insects feeding induced plant chemical defensive mechanism of the results found that under insect feeding conditions, a significant change in 28 proteins, of which 17 protein spots up-regulated expression , And 11 protein spots were down-regulated.The differential proteins were identified by matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF MS) and found that transketolase, S-adenosylmethionine synthetase, Octamidate dehydrogenase and fatty acid synthase play an important role in plant-induced chemical defenses, and fatty acid synthase is associated with the jasmonic acid metabolic pathway.