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目的:探讨抗M2胆碱能受体抗体(M2-AB)对T淋巴细胞的增殖与分泌功能有何影响。方法:分离培养大鼠脾脏T淋巴细胞,免疫荧光染色鉴定;CCK-8法检测经M2-AB干预后T淋巴细胞的增殖水平,并测定培养上清中IL-4与IL-10含量。结果:(1)经刀豆蛋白刺激培养72h后,CD3-FITC与CD4-PE鉴定所培养
Objective: To investigate the effect of anti-M2 cholinergic receptor antibody (M2-AB) on the proliferation and secretion of T lymphocytes. Methods: T lymphocytes from spleen of rats were isolated and identified by immunofluorescence staining. The proliferation of T lymphocytes was detected by CCK-8 assay and the levels of IL-4 and IL-10 in culture supernatants were determined. Results: (1) CD3-FITC and CD4-PE were cultured 72 hours after induced by concanavalin