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研究小檗胺（Ber）对氯化钾（KC1）和哇巴因（Oua）诱导的家兔血管平滑肌细胞（VSMC）内游离钙（［Ca(2+)］i）的影响。培养的VSMC以荧光探针Fluo-3/AM负载，通过激光共聚焦显微镜检查法检测［Ca(2+)］i。细胞外钙2．0mmol·L(-1)时，VSMC静息时［Ca(2+)］i水平为（228±46）／FI（n＝26），且不被Ber30βmol·L(-1)所影响（P＞0．05）。但KCl60mmol.L(-1)升高的[Ca(2+)]i；可被Ber预处理所抑制（P＜0.01）。荧光强度达峰值的时间明显延长（P＜0．05或P＜0．01）。实验结果还显示，在标本中加入KC1或Oua，VSMC核内的荧光值不受影响。Ber的这些作用与Ver10μmoh.L(-1)的作用相似。本研究的结果显示，Ber可拮抗KC1或Oua升高［Ca(2+)］的作用，此抑制作用可能与Ber阻断电压依赖性钙通道及激活Na+－K+－ATPase的作用有关。
To investigate the effect of berberine on free calcium ([Ca (2 +)] i) in rabbit vascular smooth muscle cells (VSMC) induced by potassium chloride (KCl) and ouabain (Oua) The cultured VSMCs were loaded with fluorescent probe Fluo-3 / AM and [Ca (2 +)] i was detected by laser scanning confocal microscopy. The level of [Ca (2 +)] i at resting VSMC was (228 ± 46) / FI (n = 26) when cells were treated with extracellular calcium 2.0 mmol·L -1 ) (P> 0.05). However, [Ca (2 +)] i increased with KCl up to 60mmol.L (-1) and was inhibited by Ber pretreatment (P <0.01). Fluorescence intensity peaked significantly longer (P <0.05 or P <0.01). The experimental results also showed that the addition of KC1 or Oua in the specimen did not affect the fluorescence value in the VSMC nucleus. These effects of Ber are similar to those of Ver10 μmoh.L (-1). The results of this study show that Ber can antagonize the increase of [Ca (2+)] by KC1 or Oua, which may be related to Ber’s action of blocking voltage-dependent calcium channels and activating Na + -K + -ATPase.