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本实验应用健康大白鼠20只,借助立体定位仪及微电泳导入20％HRP到大白鼠缝际大核内。在导入HRP后立即拔出电极者或动物存活超过60小时者,其脑内找不到外源性HRP颗粒,而在导入HRP后,再停留电极10分以上,动物存活40小时者则可以见到外源性HRP颗粒分布于不同脑水平的神经元内:其中以延髓网状结构的多突神经元最多,最明显;在桥脑室底灰质、网状结构、蓝斑的腹外侧部等处,偶而可以见到小的梭形神经元内有阳性反应颗粒,中脑导水管周围灰质,缝际正中上核,黑质致密部,视上核的周缘部也能见到一些细胞内含有弱阳性反应颗粒。此外,在丘脑及皮质下结构内皆未找到有阳性颗粒反应的细胞。在网状结构及室底灰质的某些血管周缘可见到有两种HRP阳性反应细胞:其一似为小的原浆性星形胶质细胞,核小,胞质宽,具有短而粗的不规则胞突;另一为多突神经元,在其核周充满了棕色阳性反应颗粒,其胞突内HRP颗粒呈串珠状排列。
In this experiment, 20 healthy rats were used to introduce 20% HRP into the interphalangeal nucleus of rats by stereotaxic instrument and microelectrophoresis. When HRP was pulled out immediately or the animals survived for more than 60 hours, exogenous HRP particles could not be found in the brain, and after HRP was introduced, the electrodes were left for more than 10 minutes and the animals survived for 40 hours Exogenous HRP particles distributed in neurons of different brain levels: in which the majority of neurons in the medulla oblongata structure most, most obvious; in the pons in the pontine gray matter, reticular structure, the blue spot of the ventrolateral Department , And occasionally you can see a small spindle neurons positive particles, the midbrain periaqueductal gray, the middle of the interocular nucleus, substantia nigra compact part, the peripheral part of the supraoptic nucleus can also see some cells containing weak Positive reaction particles. In addition, no positive particle-responsive cells were found in the thalamus and subcortical structures. There are two types of HRP-positive cells visible on the periphery of the reticular structures and the gray matter of the fundal compartment: one is a small, prodromal astrocyte with a small nucleus, a wide cytoplasm, short and thick Irregular cell process; the other is multi-neuron, full of brown positive reaction particles in its perinuclear weeks, the intra-cellular HRP particles beaded arrangement.