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目的 研究传染性非典型肺炎 (SARS)重型和普通型患者外周血淋巴细胞亚群异常变化 ,以期完善诊断标准。方法 采用多色荧光标记流式细胞术结合血液分析仪检测 155例SARS患者的外周血淋巴细胞及其亚群的表达情况。结果 普通型组与重型组比较 ,外周血白细胞、淋巴细胞、CD3 +CD4+、CD3 +CD4-CD8-、NK、CD19+CD5+、CD45RA +CD4+、CD45RO +CD4+、CD2 8+CD4+、CD2 8+CD8+、CD2 8+CD8-、CD95+CD4+等亚群的绝对值差异有显著性 ,两组均低于健康人 ,且重型组低于普通型组。结论 SARS患者的大部分淋巴细胞及亚群均受到破坏 ,以T淋巴细胞功能减低最为显著。外周血淋巴细胞各亚群的绝对值和百分比对于反映SARS患者免疫功能的改变情况均有意义。外周血淋巴细胞各亚群检测有望成为新的SARS实验诊断与监测指标
Objective To study the abnormal changes of peripheral blood lymphocyte subsets in patients with severe and common type of SARS and to improve the diagnostic criteria. Methods The expression of peripheral blood lymphocytes and their subpopulations in 155 cases of SARS were detected by multi-color fluorescent-labeled flow cytometry and hematology analyzer. Results Compared with the severe group, the levels of leukocyte, lymphocyte, CD3 + CD4 +, CD3 + CD4-CD8-, NK, CD19 + CD5 +, CD45RA + CD4 +, CD45RO + CD4 +, CD28 +, CD28 + CD8 + , CD2 8 + CD8-, CD95 + CD4 + and other subgroups were significantly different, both groups were lower than healthy people, and the severe group was lower than the common type group. Conclusion Most of the lymphocytes and their subpopulations in patients with SARS were damaged, and the most significant reduction of T lymphocyte function was found. The absolute value and percentage of each subsets of peripheral blood lymphocytes are meaningful for reflecting the changes of immune function in patients with SARS. Peripheral blood lymphocyte subsets detection is expected to become a new SARS laboratory diagnosis and monitoring indicators