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目的:观察参苏饮对炎症人支气管上皮细胞(16HBE)表达β-防御素2(h BD-2)的影响。方法:将40只SPF级SD雄性大鼠灌胃给药参苏饮及其拆方,心脏采血制备含药血清,传代培养人支气管上皮细胞(16HBE),利用1mg/L LPS刺激12h建立体外16HBE细胞炎症模型后分为5组,即空白对照组、模型组、参苏饮全方组、益气解表组和止咳化痰组。Western法检测给药3h、8h后各组胞浆蛋白h BD-2的表达情况。结果:与空白对照组比较,模型组h BD-2蛋白含量在给药3h显著升高(P<0.05),而在给药8h两组比较差异无统计学意义(P>0.05);与模型组比较,参苏饮全方组、益气解表组、止咳化痰组在给药3h、8h均能显著性上调h BD-2蛋白含量(P<0.05)。结论:参苏饮能够促进炎症人支气管上皮细胞表达h BD-2蛋白,提示参苏饮发挥益气固表、抗菌消炎的物质基础可能与h BD-2有关。
Objective: To observe the effects of Shen Su Yin on expression of β-defensin 2 (h BD-2) in inflammatory human bronchial epithelial cells (16HBE). Methods: Forty SPF SD male rats were orally administered with Shen Su Yin and its decomposed formulas. Blood was collected from the heart to prepare serum containing human bronchial epithelial cells (16HBE). The cultured human bronchial epithelial cells (16HBE) were stimulated with 1 mg / L LPS for 12 h to establish 16HBE in vitro The model of inflammatory cells was divided into 5 groups: blank control group, model group, ShenShen Decoction group, YiQiXingTiao group and cough and phlegm group. The Western blot was used to detect the expression of h BD-2 in each group after administration for 3h and 8h. Results: Compared with the blank control group, the protein content of h BD-2 in model group was significantly increased at 3h (P <0.05), while there was no significant difference between the two groups at 8h after administration (P> 0.05) Compared with the control group, the content of h BD-2 protein increased significantly (P <0.05) at 3 h and 8 h after administration of ShenShen Decoction, YiQiXuTiao Decoction and ZhiChi Decoction. Conclusion: Ssu-Sinken can promote the expression of h BD-2 protein in inflammatory human bronchial epithelial cells, suggesting that Ssu-Ssu-Yin can exert Yi-qi form and the substance basis of anti-bacterial and anti-inflammatory may be related to h BD-2.