论文部分内容阅读
以 NSE 的 cDNA 制备基因探针,用 Northern blot 方法检测 NSE mRNA 的相对含量,观察碘缺乏(甲低)对大鼠 NSE 基因表达的影响,结果表现1日龄和20日龄低碘甲低大鼠 NSE mRNA 含量均低于同龄正常大鼠,表明低碘甲低可于转录水平阻碍 NSE 的基因表达。
NSE mRNA was used to prepare the gene probe. Northern blot was used to detect the relative content of NSE mRNA. The effect of iodine deficiency (hypothyroidism) on NSE gene expression in rats was observed. The results showed that on the 1st and 20th day, NSE mRNA levels in rats were lower than normal rats of the same age, indicating that hypothyroidism can inhibit gene expression of NSE at the transcriptional level.