肝门部胆管癌是消化系统中恶性程度最高的肿瘤之一,术后复发率高,预后极差~([1-2])。其根本原因在于肝门部胆管癌早诊率低,就诊时大部分患者既已失去手术机会,而且胆管癌侵袭能力强、转移率高~([3-4])。其中神经侵犯是肝门胆管癌的一大特征,胆管癌的神经侵犯率高达80%~90%~([5-7])。为了深入研究胆管癌神经侵犯的病理发生机制,合适的模型是关键环节。本 Hilar cholangiocarcinoma is one of the most malignant tumors in the digestive system, with a high recurrence rate and poor prognosis (1-2). The fundamental reason is the low rate of early diagnosis of hilar cholangiocarcinoma, the majority of patients treated at the time of surgery have lost the opportunity, and the invasion of bile duct cancer strong, high transfer rate ~ ([3-4]). Nerve invasion of which is a major feature of hilar cholangiocarcinoma, nerve invasion rate of cholangiocarcinoma as high as 80% to 90% ~ ([5-7]). In order to further study the pathological mechanism of nerve invasion of cholangiocarcinoma, the appropriate model is the key link. this