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目的分析2011年-2014年武威市腹泻住院儿童粪便中病毒性感染的情况和变化,掌握地区儿童病毒性腹泻优势病毒型别,为今后腹泻住院儿童治疗和预防提供支持。方法采集武威市凉州区医院5岁以下住院腹泻儿童粪便,每月采集10份~30份样品,采用ELISA方法进行A组轮状病毒初筛,后用WHO推荐的多重RT-PCR法进行分型。结果甘肃省武威市2011年-2014年腹泻住院儿童感染病毒性腹泻优势病原中,A组轮状病毒依然是造成儿童病毒性腹泻最主要病原体,其次为杯状病毒,腺病毒所占比例总体不高,2014年星状病毒比例明显上升;A组轮状病毒分型由2011年G1P8、G3P8为主,经过2年G9P8分型逐渐成为流行优势型别,并形成较稳定的流行。结论武威市病毒性腹泻病原分型特点较全国大部分地区流行情况大体一致,但也有其特有规律。
Objective To analyze the situation and changes of viral infections in the stools of children hospitalized in diarrhea in Wuwei City from 2011 to 2014. To master the genotypes of virus predominant in children with viral diarrhea in the area and provide support for the future treatment and prevention of diarrhea hospitalized children. Methods The feces of children under 5 years of age hospitalized with diarrhea in Wuzhou Liangzhou District Hospital were collected, and 10 to 30 samples were collected every month. The group A rotavirus was screened by ELISA and then multiplexed by WHO RT-PCR type. Results Among the predominant pathogens of viral diarrhea in hospitalized children with diarrhea in Wuwei City, Gansu Province, from 2011 to 2014, group A rotavirus was still the most important causative agent of viral diarrhea in children, followed by calicivirus. The overall proportion of adenovirus was not High, the proportion of astrovirus increased significantly in 2014; Group A rotavirus typing in 2011 G1P8, G3P8-based, after 2 years of G9P8 typing has become a predominant genotype, and the formation of a more stable epidemic. Conclusion The prevalence of viral diarrhea in Wuwei City is more or less the same as the prevalence in most parts of the country, but there are also some peculiar laws.