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目的检测子痫前期患者胎盘组织中RECK、基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)的基因表达,探讨它们与胎盘滋养细胞浸润过程的调控关系。方法采用RT-PCR、Western blotting、免疫组织化学检测120例妊娠足月剖宫产(正常妊娠、轻度子痫前期、中度子痫前期、重度子痫前期各30例)胎盘组织中RECK、MMP-9、VEGF的基因表达。结果 3组子痫前期患者胎盘组织中MMP-9及VEGF的mRNA表达水平均显著低于正常妊娠组(P<0.05);重度子痫前期组中RECK mRNA的表达显著高于正常妊娠组(P<0.05);3组子痫前期患者胎盘组织中MMP-9及VEGF蛋白表达均显著低于正常妊娠组(P<0.05),中度和重度子痫前期组中RECK蛋白表达显著高于正常妊娠组(P<0.05)。结论子痫前期患者胎盘中RECK与MMP-9、VEGF之间存在负相关性,它们可能参与了胎盘滋养细胞浅浸润过程的调控。
Objective To detect the gene expression of RECK, MMP-9 and VEGF in placenta of preeclampsia and to explore their relationship with the regulation of placental trophoblast invasion. Methods RT-PCR, Western blotting and immunohistochemistry were used to detect the expression of RECK in 120 cases of cesarean section (normal pregnancy, 30 cases of mild preeclampsia, 30 cases of moderate preeclampsia and severe preeclampsia) MMP-9, VEGF gene expression. Results The mRNA expression levels of MMP-9 and VEGF in placenta of preeclampsia group were significantly lower than those in normal pregnancy group (P <0.05). The expression of RECK mRNA in severe preeclampsia group was significantly higher than that in normal pregnancy group <0.05). The expression of MMP-9 and VEGF in placenta of preeclampsia patients was significantly lower than that of normal pregnancy group (P <0.05), and the expression of RECK protein in moderate and severe preeclampsia group was significantly higher than that of normal pregnancy Group (P <0.05). Conclusion There is a negative correlation between RECK and MMP-9 and VEGF in the placenta of preeclampsia, which may be involved in the regulation of shallow infiltration of placental trophoblast cells.