论文部分内容阅读
目的：观察Ｖｅｒａｐａｍｉｌ（异搏定）对体外培养角膜基质细胞的影响，为其临床应用提供基础。方法：进行兔角膜基质细胞的原代和早期传代培养，并用ＭＴＴ自动比色法检测Ｖｅｒａｐａｍｉｌ对兔角膜基质细胞增殖的影响。结果：Ｖｅｒａｐａｍｉｌ浓度１０～１０００μｇ／ｍｌ作用４８ｈ和７２ｈ对角膜基质细胞增殖有明显抑制作用（Ｐ＜０．０５）。作用７２ｈ抑制率高于作用４８ｈ。结论：Ｖｅｒａｐａｍｉｌ为剂量依赖型和时间依赖型药物，能有效地抑制角膜基质细胞增殖，可望成为调节角膜伤口愈合的新药物。
Objective: To observe the effect of Verapamil on cultured corneal stromal cells in vitro and provide a basis for its clinical application. Methods: Primary and early subcultures of rabbit corneal stromal cells were performed. The effects of Verapamil on the proliferation of rabbit corneal stroma cells were detected by MTT assay. Results: Verapamil at a concentration of 10 ~ 1000μg / ml for 48h and 72h significantly inhibited corneal stromal cell proliferation (P <0.05). 72h inhibition rate higher than the role of 48h. Conclusion: Verapamil is a dose-dependent and time-dependent drug, which can effectively inhibit the proliferation of corneal stromal cells and is expected to become a new drug that regulates corneal wound healing.