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目的研究太空诱变对α-溶血性链球菌多糖分子表达的影响。方法采用改良的过碘酸钠法将辣根过氧化物酶(HRP)分别标记在刀豆凝集素(ConA)、麦胚凝集素(WGA)、接骨木凝集素(SNA)、蓖麻凝集素Ⅰ(RCA-Ⅰ)及植物血凝素(PHA)上,合成HRP-凝集素,检测普通型α-溶血性链球菌及太空型链球菌菌体内多糖分子的表达水平。结果太空型链球菌菌体内末端为ConA、SNA及RCA-Ⅰ所识别的多糖分子含量较普通型α-溶血性链球菌高,且差异有统计学意义;末端为WGA及PHA所识别的多糖分子含量,普通型α-溶血性链球菌较太空型链球菌稍高,但差异无统计学意义。结论太空诱变对细菌的多糖分子表达具有调控作用,为细菌多糖制品的筛选及其性能和产量的提高提供了新的途径。
Objective To study the effect of space mutagenesis on the expression of α-hemolytic streptococcus polysaccharide molecules. Methods The horseradish peroxidase (HRP) was labeled with ConA, WGA, SNA, Ricinus communis agglutinin Ⅰ (RCA-Ⅰ) and phytohemagglutinin (PHA), HRP-agglutinin was synthesized to detect the expression level of polysaccharide molecules in common α-hemolytic streptococcus and Streptococcus pneumoniae. Results The content of polysaccharides recognized by ConA, SNA and RCA-Ⅰ in S. aureus was higher than that of common hemolytic streptococcus, and the difference was statistically significant. The end was polysaccharide molecules recognized by WGA and PHA Content, common type α-hemolytic streptococcus slightly higher than the space-type streptococcus, but the difference was not statistically significant. Conclusions Space mutagenesis can regulate the expression of bacterial polysaccharides, which provides a new approach for the screening of bacterial polysaccharide products and the improvement of its performance and yield.