论文部分内容阅读
目的:检测GnRHⅡ蛋白在子宫内膜异位症患者异位子宫内膜、在位子宫内膜和正常子宫内膜中的表达情况,同时分析其表达是否与子宫内膜月经周期有关。方法:采用免疫组织化学SP法检测GnRHⅡ蛋白在异位内膜、在位内膜及正常子宫内膜组织中的表达情况,并分析和比较其表达是否有差异。结果:GnRHⅡ蛋白在子宫内膜异位症患者异位、在位子宫内膜及正常子宫内膜中均有表达,阳性表达定位于子宫内膜腺体及间质细胞的细胞质;GnRHⅡ蛋白在异位内膜、在位内膜及对照组正常内膜的表达依次增强,两两比较差异有统计学意义(P<0.05);GnRHⅡ蛋白在正常子宫内膜分泌期表达强于增生期(P<0.05),且以分泌早中期最强,显著强于增生期和分泌晚期(P<0.01),而异位组或在位组的分泌期与增生期比较,差异无统计学意义(P>0.05)。结论:GnRHⅡ蛋白在子宫内膜异位症的发病中以及在人类月经生理方面可能起重要作用。
Objective: To detect the expression of GnRHⅡ in ectopic endometrium, endometriosis and normal endometrium in patients with endometriosis, and to analyze whether the expression of GnRHⅡ is related to the endometrial menstrual cycle. Methods: Immunohistochemical SP method was used to detect the expression of GnRHⅡprotein in ectopic, eutopic and eutopic endometrium, and to analyze and compare the expression of GnRHⅡprotein. Results: The GnRHⅡ protein was expressed in ectopic endometrium of endometriosis and normal endometrium, and the positive expression of GnRHⅡ protein was localized in the cytoplasm of endometrial gland and interstitial cells. The expression of GnRHⅡ in endometrium, eutopic endometrium and normal endometrium was enhanced in turn, and the difference was statistically significant (P <0.05). The expression of GnRHⅡ protein in normal endometrium was stronger than that in proliferative phase (P < 0.05). There was no significant difference between the ectopic group and the eutopic group or the eutopic group and the proliferative phase in the ectopic group or in the eutopic group (P> 0.05) ). Conclusion: GnRH Ⅱ protein may play an important role in the pathogenesis of endometriosis and in the physiology of human menstruation.