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牡丹黑斑病的致病菌尚不明确。通过对泰安地区的牡丹病叶进行组织分离,利用分子生物学技术结合形态学方法对病原菌进行鉴定,并利用Mega5软件对链格孢属的22个种进行系统发育树分析,进一步明确其分类地位。结果表明:该病致病菌ITS序列与GenBank中登录的Alternaria alternata、A.tenuissima、A.metachromatica相似性均为100%,利用DNAMAN软件对核苷酸一致性进行比较,发现样品菌株与A.alternata的一致性最高,结合形态学方法鉴定该病致病菌为链格孢A.alternata(GenBank登录号为:KJ682317)。回接鉴定后分离得到的菌株,菌饼刺伤接种的病叶与无伤接种的病叶均发病,但刺伤叶片病斑扩展快。
Peony black spot pathogens is not yet clear. Pathogens were identified by molecular biology techniques combined with morphological methods by tissue isolation of diseased leaves of tree peony in Tai’an. Phylogenetic tree analysis of 22 species of Alternaria was carried out by using Mega5 software to further clarify its taxonomic status . The results showed that the ITS sequences of the pathogen were 100% identical to those of Alternaria alternata, A. tenuissima and A. metachromatica registered in GenBank. Nucleotide identities were compared using DNAMAN software. The highest identity of alternata was confirmed by morphological method. The pathogen was A. certernata (GenBank accession number: KJ682317). Back to the identification of isolated strains, diseased leaves interstitial cake inoculated with non-injury inoculated diseased leaves are diseased, but the stab leaf lesions expanded rapidly.