论文部分内容阅读
目的观察Pax9对小鼠帽状期和钟状期牙胚牙乳头细胞的作用。方法培养胚胎14.5d和16.5d小鼠下颌第一磨牙牙胚的牙乳头细胞。转染Pax9 siRNA至牙乳头细胞中敲低Pax9的表达,CCK8检测转染后24、48、72和96h细胞的增殖情况,Real-time PCR检测转染后Msx1、Bmp2、Bmp4的表达变化。在转染Pax9 siRNA的同时进行矿化诱导培养7d,然后检测Alp、Dmp1和Dspp的表达情况。结果敲低Pax9表达后,牙乳头细胞的增殖能力减弱;Msx1的表达水平下降,而Bmp2和Bmp4的表达水平升高;牙本质形成相关基因-Alp、Dmp1和Dspp的表达水平升高,牙乳头细胞的矿化能力增强。结论 Pax9参与调控小鼠帽状期和钟状期牙乳头细胞的增殖和成牙本质分化,同时调控下游基因Msx1、Bmp2和Bmp4的表达。
Objective To observe the effect of Pax9 on the dental papilla cells of the capillaries and bell phase in mice. Methods Culture the dental papilla cells of the mandibular first molar tooth germ at 14.5 and 16.5 days. Pax9 siRNA was transfected into dental papilla cells to knock down the expression of Pax9. CCK8 was used to detect the proliferation of cells at 24, 48, 72 and 96 hours after transfection. The expression of Msx1, Bmp2 and Bmp4 was detected by Real-time PCR. Pax9 siRNA was transfected simultaneously with mineralization-induced culture for 7 days, and then the expression of Alp, Dmp1 and Dspp was detected. Results The expression of Pax9 knocked down the proliferation of dental papilla cells; the expression of Msx1 decreased, while the expression of Bmp2 and Bmp4 increased; the expressions of genes related to dentin formation-Alp, Dmp1 and Dspp increased, Cell mineralization enhanced. Conclusion Pax9 is involved in the regulation of proliferation and odontoblast differentiation of mouse papillary and bell-shaped dental papilla cells and the expression of Msx1, Bmp2 and Bmp4.