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从V20B/花1B杂交后代中发现了水稻小穗分化受阻的突变体fzp.fzp株叶形态正常,但植株分蘖数明显减少,最显著的变异是fzp植株的小穗分化完全被阻断,在正常植株枝梗分化为小穗的部位,fzp植株却形成一团枝梗.遗传分析表明,fzp受一对隐性基因控制,其相应基因拟名为fzp(t).显然.fzp(t)是控制小穗分化的关键基因.在一些F2群体中,因遗传背景发生改变,部分突变型植株表现为“中间类型”,推测可能是冗余基因或其他修饰基因、互作基因的作用.采用微卫星标记技术和BSA分析方法,将突变基因fzp(t)定位于第7染色体上的长臂末端,其中RM172和RM248位于fzp(t)一侧,它们与fzp(t)的遗传图距分别为3.3和6.4 cM;RM18和RM234位于fzp(t)的另一侧,与fzp(t)的遗传距离分别为23.1和25.3 cM.研究结果为进一步对该基因的克隆和功能研究奠定了基础.
From the progeny of V20B / Hua 1B hybrids, we found that the mutant of fzp.fzp with obstructed spike differentiation was normal but the number of tillers decreased obviously. The most significant variation was that the spike differentiation of fzp plants was completely blocked. Fzp (fzp (t)), fzp (t), fzp (t), fzp (t), fzp Is a key gene controlling spike differentiation.In some F2 populations, some of the mutant plants showed “intermediate type” due to genetic background change, suggesting that they may be redundant genes or other modified genes and interacting genes. The fzp (t) gene was located on the long arm end on chromosome 7 with microsatellite markers and BSA analysis. Among them, RM172 and RM248 were located on the fzp (t) side and their genetic distances to fzp (t) 3.3 and 6.4 cM, respectively.The RM18 and RM234 were located on the other side of fzp (t) with the genetic distance of fzp (t) of 23.1 and 25.3 cM, respectively.The results of the study laid the foundation for further study on the cloning and function of this gene.