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以羊肝细粒棘球蚴囊液抗原免疫BaLb/c鼠脾细胞与SP2/o骨髓瘤细胞常规方法融合。用人肝棘球蚴囊液抗原间接ELISA法检测,获得3株稳定分泌抗体的杂交瘤细胞株,2G_4、2F_1和2F_(10)。对人肝包囊液抗原ELISA的终点分别为1∶204800,1∶819200和1∶3200。对纯化羊肝包囊液抗原致敏羊血球IHA的终点均为1∶512,但3株单抗等量混合后IHA滴度达1∶4096。对猪囊尾蚴抗原的ELISA滴度分别为1∶4000、1∶2000和1∶2000。对泡球蚴抗原ELISA滴度分别为1∶2000、1∶1000和1∶2000。交叉阻断ELISA试验的结果初步显示此3株单抗可能识别不同的抗原表位。3株细胞在液氮中冻存两年复苏后,仍继续稳定分泌抗体。
The spleen cells of BaLb / c mice were immunized with the conventional method of SP2 / o myeloma cells by immunizing sheep cytoplasm with Echinococcus granulosus solution. Three strains of hybridoma cell lines stably secreting antibodies, 2G_4, 2F_1 and 2F_ (10) were obtained by indirect ELISA with human hepatocysts hydatid cyst fluid antigen. The end points of the human hepatocytic fluid antigen ELISA were 1:204800, 1:819200 and 1:3200, respectively. The end points of sheep blood cells IHA sensitized with purified goat liver cyst fluid antigen were all 1: 512, but the IHA titer of the 3 McAbs was 1:4096 after mixing in the same amount. ELISA titers against Cysticercus cellulosae antigen were 1: 4000, 1: 2000 and 1: 2000, respectively. ELISA titers of 1%, 1: 1000, and 1: 2000 against the bacterium of the cysticerci. The results of cross-blocking ELISA test showed that the three McAbs may recognize different epitopes. After three strains of cells were frozen in liquid nitrogen for two years, they continued to secrete antibodies steadily.