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目的探讨四种梅毒血清学实验诊断方法的准确性及在临床的应用价值。方法应用甲苯胺红不加热血清学试验(TRUST)、酶联免疫吸附试验(ELISA)和胶体金法检测518例性病科患者血清,并用梅毒螺旋体明胶颗粒凝集试验(TPPA)确认。结果 TRUST法灵敏度为58.18%,ELI SA法为99.37%、胶体金法为99.06%;TRUST法特异度为88.5%,ELI SA法为98.50%、胶体金法为100%。结论梅毒检测方法各有优缺点,应根据检验目的合理选用不同的试验方法。
Objective To investigate the accuracy and clinical value of four serological tests for syphilis. Methods Serum samples from 518 patients with STD were detected by TRUST, ELISA and colloidal gold assay, and confirmed by TPPA. Results The sensitivity of TRUST was 58.18%, that of ELI SA was 99.37%, that of colloidal gold was 99.06%, that of TRUST was 88.5%, that of ELI SA was 98.50%, and that of colloidal gold was 100%. Conclusion Syphilis detection methods have their own advantages and disadvantages, should be based on the rational use of different test methods.