论文部分内容阅读
为研究不同类型反流对食管上皮细胞增殖及DNA倍体的影响在食管肿瘤诱发过程中的作用 ,制作单纯胃食管反流 (G组 )、单纯十二指肠食管反流 (D组 )、胃十二指肠混合液食管反流 (DG组 )及无反流对照 (C组 )大鼠模型 ,于术后 4周开始腹腔内注射食管致癌剂甲基戊基亚硝胺 (MANA) ,5mg/kg ,1次 /周 ,共 15周。于术后 2 0、2 6、40周分批取出食管标本。用SABC法免疫组化染色检测食管上皮中增殖细胞核抗原 (PCNA)的表达。制备食管粘膜组织单细胞悬液 ,经DNA染色后行流式细胞仪检测 ,分析样品的DNA指数 (DI)、增值指数(PI)及DNA非整倍体率。结果显示 ,各组的PCNA标记指数 (LI)均随时间延长而显著增加 ,各时间点反流组的LI增加均较对照组更为明显 ,D、DG组又较G组高。随反流和诱癌时间的延长各组食管上皮的平均DI及PI亦逐渐增加 ,非整倍体率逐渐增高 ,反流各组的增加程度比C组显著更高 ,其中D组、DG组最为显著。提示各种类型食管反流均可致食管上皮细胞增殖异常及DNA含量异常 ,并促进细胞恶性增殖的发生 ,有十二指肠内容物反流的组内细胞增殖异常更为明显。细胞增殖异常与食管肿瘤的发生率有密切关系
To study the effects of different types of reflux on esophageal epithelial cell proliferation and DNA ploidy in esophageal tumor induction, simple gastroesophageal reflux (group G), simple duodenoesophageal reflux (group D), Gastroduodenal mixed esophageal reflux (DG group) and non-reflux control (C group) rat models began intraperitoneal injection of the esophageal carcinogen methyl nitrosamine (MANA) 4 weeks after surgery. 5mg/kg, 1 time/week for 15 weeks. Esophageal specimens were removed in batches at 20, 26, and 40 weeks after surgery. The expression of proliferating cell nuclear antigen (PCNA) in esophageal epithelium was detected by SABC immunohistochemical staining. A single cell suspension of esophageal mucosal tissue was prepared and subjected to flow cytometry after DNA staining. The DNA index (DI), value-added index (PI), and DNA aneuploidy were analyzed. The results showed that the PCNA labeling index (LI) of each group increased significantly with time. The increase in LI at each time point in the reflux group was more significant than that in the control group. The D and DG groups were higher than those in the G group. The average DI and PI of the esophageal epithelium increased gradually with the time of reflux and prolongation of cancer induction, and the aneuploidy rate gradually increased. The degree of increase of reflux in each group was significantly higher than that in group C, including group D and group DG. The most significant. All kinds of esophageal reflux can cause abnormal proliferation of esophageal epithelial cells and abnormal DNA content, and promote the occurrence of malignant proliferation of cells. The abnormal cell proliferation in the group with duodenal reflux is more obvious. Abnormal cell proliferation is closely related to the incidence of esophageal cancer