我们采用30％聚乙二醇(PEG,分子量为6,000)作为人类绒毛膜促性腺激素(HCG)放射免疫测定中,结合标记抗原和游离标记抗原的分离剂。同时对60份血清标本,应用本法和双抗体法进行比较,结果两种方法无显著差异,且有非常显著的相关。现报道如下。 材料和方法 一、试剂:HCG标准液:~125I-HCG标记液;HCG血清(以上3种试剂由上海生物制品研究所供);30％PEG溶液(分子量为6,000)。 二、方法:见表1。 We use 30% polyethylene glycol (PEG, molecular weight 6,000) as a separating agent for binding labeled and free labeled antigens in human chorionic gonadotropin (HCG) radioimmunoassay. At the same time on 60 serum samples, the application of this method and the double antibody method for comparison, the results of two methods no significant difference, and there is a very significant correlation. Report as follows now. Materials and Methods 1. Reagents: HCG standard solution: ~ 125I-HCG labeling solution; HCG serum (the above three reagents are provided by Shanghai Institute of Biological Products); and 30% PEG solution (molecular weight is 6,000). Second, the method: see Table 1.