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探讨抗氧化剂N-乙酰-L-半胱氨酸(NAC)对缺氧缺血新生大鼠海马CAl区神经元凋亡的影响。将70只7日龄Wistar大鼠随机分成3组,①假手术组:(n=10)仅作颈正中切口,不作颈总动脉结扎;②观察组(NAC组n=30,HIBD)后即刻腹腔内注射NAC 200ug/只;③对照组(生理盐水组n=30),HIBD后即刻腹腔注射等体积的生理盐水。假手术组于手术后即刻,观察组、对照组于处置后48小时断头取脑,进行HE和Tunel染色光镜下检测脑细胞凋亡数。结果:对照组大风海马CAl区可见典型的凋亡细胞,表现为细胞固缩、胞浆深染、核浓缩、碎片、核浆分布不清,有凋亡小体形成;NAC组上述凋亡细胞明显减少;Tunel染色对照组阳性细胞数与NAC组比较差异有明显的统计学意义(P<0.05)。结论:NAC对HIBD后海马CAl区神经细胞凋亡有明显的抑制作用。
To investigate the effect of antioxidant N-acetyl-L-cysteine (NAC) on neuronal apoptosis in hippocampus CA1 area of hypoxic-ischemic neonatal rats. Seventy seven-day-old Wistar rats were randomly divided into three groups: ① sham-operation group: (n = 10) only for mid-neck incision, without carotid artery ligation; ② in observation group (n = 30, HIBD) Intraperitoneal injection of NAC 200ug / only; ③ control group (n = 30 saline group), immediately after HIBD intraperitoneal injection of equal volume of saline. Immediately after the operation, the observation group and the control group were decapitated and the brains were harvested at 48 hours after treatment. The apoptosis of brain cells was detected by HE and Tunel staining. Results: Apoptotic cells were observed in the CA1 area of the windy-wind hippocampus in the control group, which were characterized by cell shrinkage, deep staining of cytoplasm, condensation of nucleus, fragmentation and unclear distribution of nuclear plasma and formation of apoptotic bodies. (P <0.05). There was significant difference between Tunel staining control group and NAC group (P <0.05). Conclusion: NAC can significantly inhibit the apoptosis of hippocampal CA1 neurons in HIBD patients.