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目的检测睾酮作用下雄激素依赖性前列腺癌细胞LNCaP中雄激素反应性基因(ARGs)的表达情况。方法检测不同浓度的睾酮对雄激素依赖性前列腺癌细胞LNCaP增殖的影响,采用基因芯片确定LNCaP细胞中受睾酮调控的基因。结果低浓度的睾酮促进LNCaP细胞的生长,并呈剂量依赖性,相反较高浓度的睾酮能够抑制LNCaP的增殖。在96个与前列腺癌有关的基因中,受睾酮刺激有19个基因表达上调,8个基因表达下调。结论睾酮诱导的雄激素反应性基因在前列腺癌的发展中起非常重要的作用,基因芯片有助于高通量分析基因表达水平的变化,为前列腺癌的研究提供了非常理想的工具。
Objective To detect the expression of androgen-responsive genes (ARGs) in androgen-dependent prostate cancer cells LNCaP by testosterone. Methods The effects of different concentrations of testosterone on the proliferation of androgen-dependent prostate cancer cell line LNCaP were detected. Genes were used to determine the genes regulated by testosterone in LNCaP cells. Results Low concentrations of testosterone promoted the growth of LNCaP cells in a dose-dependent manner, whereas higher concentrations of testosterone inhibited the proliferation of LNCaP cells. Of the 96 prostate cancer-related genes, 19 were up-regulated by testosterone and 8 were down-regulated. CONCLUSION: Testosterone-induced androgen-responsive genes play a very important role in the development of prostate cancer. Gene chips facilitate high-throughput analysis of gene expression and provide a very ideal tool for the study of prostate cancer.