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目的:观察密闭舱内亚致死剂量爆炸伤大鼠血浆和额叶皮质内5-羟色胺(5-HT)的变化及其对大鼠旷场行为的影响。方法:采用在密闭舱内亚致死剂量爆炸伤的大鼠模型,高效液相色谱电化学法分析爆炸伤后不同时间点大鼠血浆和额叶皮质内5-HT浓度的变化,应用旷场行为观察箱观察大鼠旷场行为。结果:①舱内及舱外亚致死剂量爆炸伤大鼠血浆和脑组织内5-HT含量短暂升高后出现明显降低;②舱内爆炸伤后额叶皮质5-HT在48 h达峰值,随后下降,而舱外爆炸伤后在24 h即达峰值,随后下降;③亚致死剂量爆炸伤后大鼠旷场行为明显增加,且舱内明显高于舱外。结论:大鼠旷场行为变化与血浆5-HT浓度呈明显负相关。
OBJECTIVE: To observe the changes of 5-hydroxytryptamine (5-HT) in the plasma and frontal cortex in a rat model of subacute lethal dose-of-explosion and its effect on the open-field behavior in rats. Methods: Rat model of sub-lethal dose explosion injury was established in a closed chamber. The changes of 5-HT concentration in rat plasma and frontal cortex at different time points after explosive injury were analyzed by high performance liquid chromatography-electrochemical method. The open field behavior Observation box to observe the open field behavior of rats. Results: ①The contents of 5-HT in plasma and brain tissues of rats exposed to sub-lethal dose explosion in cabin were significantly decreased after transient increase; ② 5-HT in frontal cortex peaked at 48 h after explosion in cabin, And then decreased. However, the explosion peaked at 24 h after the extracavity explosion and then decreased; ③ After the sublethal dose explosion injury, the open-field behavior of the rats significantly increased, and the cabin was significantly higher than the extracavity. CONCLUSION: The change of open field behavior in rats is negatively correlated with the plasma 5-HT concentration.