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建立了Fe(Ш)-磺基水杨酸褪色光度法测定甲巯咪唑的方法。结果表明,在酸性介质中,甲巯咪唑分子中的巯基(-SH)可将Fe3+还原为Fe2+,采用磺基水杨酸作为Fe3+的显色剂,借褪色分光光度法间接测定了甲巯咪唑含量。显色体系最大吸收波长为480nm,甲巯咪唑质量浓度在0.072～0.156 mg/mL范围内与吸光度A呈良好的线性关系,线性回归方程为A=-0.1548ρ+0.0949,线性相关系数r=0.9988。方法用于实际药品中甲巯咪唑的含量测定,结果与药典法一致。
A method for the determination of methimazole by Fe (Ⅲ) -sulfosalicylic acid fading spectrophotometry was established. The results showed that the sulfhydryl (-SH) in the methimazole molecule could reduce Fe3 + to Fe2 + in acid medium, and the sulfosalicylic acid as the chromogenic reagent in Fe3 +, the methimazole was indirectly determined by fading spectrophotometry content. The maximum absorption wavelength of the chromogenic system was 480 nm and the concentration of methimazole was 0.072 ~ 0.156 mg / mL. The linear regression equation was A = -0.1548ρ + 0.0949, and the linear correlation coefficient was 0.9988 . The method was applied to the determination of methimazole in the real medicine, and the result was consistent with that of the pharmacopoeia.