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目的:研究男性和女性镍铬合金烤瓷牙修复后不同时期龈沟液IL-8含量水平,了解镍铬合金在不同时期对牙龈刺激程度的性别差异。方法:采用双抗体夹心酶联免疫吸附法(ABC-ELISA)测定男女各30例镍铬合金烤瓷牙制备前、修复后1周、修复后3个月和修复后6个月烤瓷牙龈沟液IL-8总量和含量及龈沟液量。结果:两组不同时期的IL-8总量和龈沟液量之间差异有统计学意义(P<0.01);IL-8总量、龈沟液量和IL-8含量修复3月时女性组高于男性组(P<0.05)。结论:IL-8可作为评价龈组织刺激程度的指标,镍铬合金对牙龈有刺激性,女性更为明显。
OBJECTIVE: To study the levels of IL-8 in gingival crevicular fluid of men and women with Ni-Cr alloy porcelain after repairing, and to understand the gender difference of gingival irritation in different periods of Ni-Cr alloy. Methods: Before and after preparation of nickel-chromium alloy porcelain teeth of 30 male and female, the double antibody sandwich enzyme-linked immunosorbent assay (ABC-ELISA) was used to measure the gingival sulcus of porcelain teeth after one week, three months after repair and six months after repair Total IL-8 content and content of gingival crevicular fluid. Results: The total amount of IL-8 and gingival crevicular fluid in two groups at different time had significant difference (P <0.01). The total amount of IL-8, gingival crevicular fluid and IL-8 content in women Group than in the male group (P <0.05). Conclusion: IL-8 can be used as an index to evaluate the degree of gingival tissue stimulation. Ni-Cr alloy is irritating to the gums and more pronounced in women.