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目的观察神经酰胺(CER)在高糖诱导的人脐静脉内皮细胞凋亡中的作用。方法分别以流式细胞术和TUNEL法、高压液相色谱法等检测细胞凋亡、CER和酸性鞘磷酯酶(ASM)。结果(1)高糖呈浓度依赖性引起细胞内CER和酸性ASM活性增加,左旋葡萄糖无类似效应;(2)地昔帕明可抑制高糖诱导的内皮细胞凋亡,外源性(CER)可逆转其抑制作用;(3)外源性CER及酸性ASM可诱导内皮细胞凋亡;(4)高糖诱导的细胞内CER增加与高糖浓度及细胞凋亡均正相关。结论由酸性ASM水解引起的细胞内神经酰胺增加参与了高糖诱导的内皮细胞凋亡。
Objective To investigate the role of ceramide (CER) in the apoptosis of human umbilical vein endothelial cells induced by high glucose. Methods Cell apoptosis, CER and sphingosinephosphatase (ASM) were detected by flow cytometry, TUNEL and high pressure liquid chromatography respectively. (2) Desipramine could inhibit the apoptosis of endothelial cells induced by high glucose, and the exogenous (CER) (3) Exogenous CER and acidic ASM can induce endothelial cell apoptosis; (4) High glucose induced increase of intracellular CER is positively correlated with high glucose concentration and apoptosis. Conclusion The increase of intracellular ceramide induced by acidic ASM hydrolysis is involved in the apoptosis of endothelial cells induced by high glucose.