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目的分析检测血液中癌胚抗原信使核糖核酸(CEA mRNA)诊断消化系统肿瘤血行转移的应用价值。方法以41例消化系统肿瘤患者(肿瘤组)和32例非肿瘤性消化道疾病患者(对照组)为对象,应用逆转录-套式-聚合酶链反应技术检测血液标本CEA mRNA。结果肿瘤组的CEA mRNA阳性结果(20例)远高于非肿瘤组(1例)(P<0.001),CEA mRNA指标诊断消化系统肿瘤血行转移的特异性和敏感性分别为96.9%和48.8%。结论CEA mRNA是一个实用价值较高的诊断消化系统肿瘤血行转移指标,检测血液CEA mRNA有助于了解肿瘤血行转移。
Objective To analyze the value of detecting carcinoembryonic antigen messenger ribonucleic acid (CEA mRNA) in blood to diagnose hematogenous metastasis of digestive system. Methods 41 patients with digestive system tumors (tumor group) and 32 patients with non-neoplastic digestive disease (control group) were enrolled in this study. CEA mRNA was detected by reverse transcription-nested-polymerase chain reaction in blood samples. Results The positive results of CEA mRNA in the tumor group (20 cases) were much higher than those in the non-tumor group (1 case) (P <0.001). The specificity and sensitivity of CEA mRNA in the diagnosis of hematogenous metastasis of the digestive system were 96.9% and 48.8% . Conclusions CEA mRNA is a practical diagnostic indicator of hematogenous metastasis of digestive system. Detection of CEA mRNA in blood can help understand the metastasis of tumor.