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The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other ele- ments in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angio- genic activity.
The specificity of the interaction is revealed by comparing the binding probability of aptamer to other ele-ments in a series of control experiments. The specificity of the interaction is revealed by comparing the binding interaction of angiogenin and aptamer has been investigated by using AFM between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7 ± 11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer , which provided basis for further understanding the inhibition of the aptamer to angio-genic activity.