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目的:研究玫瑰花中总黄酮醇的定性定量分析方法。方法:采用薄层色谱法,使用硅胶G薄层板,以甲苯-甲酸乙酯-甲酸(10:8:1)为展开剂,3%三氯化铝乙醇溶液为显色剂,检测山柰酚与槲皮素的存在与否;采用高效液相色谱法,使用PhenomenexC_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇～0.4%磷酸(60:40)为流动相,流速1 mL·min~(-10),在360 nm波长处测定槲皮素与山柰酚的含量。结果:采用薄层色谱法可以清晰检出槲皮素与山柰酚;采用高效液相色谱法,槲皮素进样量在0.06～0.6μg范围内呈良好的线性关系(r=0.9996),平均加样回收率(n=5)为99.2%(RSD=3.9%);山柰酚进样量在0.02～0.2μg范围内呈良好的线性关系(r=0.9993),平均加样回收率(n=5)为98.5%(RSD=4.8%)。结论:建立了玫瑰花中总黄酮醇类成分的定性定量分析方法,可作为玫瑰花药材的质量控制方法。
Objective: To study the method of qualitative and quantitative analysis of total flavonol in rose flower. Methods: Thin layer chromatography (TLC) was performed on silica gel G plate with toluene-ethyl acetate-formic acid (10: 8: 1) as developing solvent and 3% aluminum trichloride ethanol The presence of phenol and quercetin was determined by HPLC using Phenomenex C 18 column (250 mm × 4.6 mm, 5 μm) with mobile phase of methanol 0.4% phosphoric acid (60:40) 1 mL · min ~ (-10). The contents of quercetin and kaempferol were determined at the wavelength of 360 nm. Results: Quercetin and kaempferol were detected clearly by TLC. The calibration curve of quercetin was linear in the range of 0.06-0.6 μg (r = 0.9996) by HPLC. The average recovery (n = 5) was 99.2% (RSD = 3.9%). There was a good linear relationship between the amount of kaempferol and 0.02 ~ 0.2μg (r = 0.9993) n = 5) was 98.5% (RSD = 4.8%). Conclusion: The method of qualitative and quantitative analysis of flavonoids in rose is established, which can be used as quality control method of rose flower.