论文部分内容阅读
光动力疗法(PDT)作为一种迅速发展的传统替代疗法,在抗癌治疗中显示出巨大的潜力。为增强靶向性和提高光催化杀伤效率,本研究设计了一种新型光敏剂Fe_3O_4-Ti O_2磁性纳米粒。在不同外磁场下,考察其在可见光和紫外光激发下对肝癌细胞的杀伤效应。同时利用流式细胞术检测纳米Fe_3O_4-Ti O_2对肝癌细胞凋亡率、细胞周期和线粒体膜电位的影响。根据纳米Fe_3O_4-Ti O_2和肝癌细胞的作用方式探讨其抗癌机制。结果表明,可见光激发纳米Fe_3O_4-Ti O_2可以杀伤癌细胞,且其杀伤效率与紫外光激发下无明显差别。此外,Fe_3O_4-Ti O_2比Ti O_2具有更高的细胞摄取率,从而使其具有更高的选择性和光催化杀伤效率。其作用机制是光催化纳米Fe_3O_4-Ti O_2产生活性氧ROS抑制癌细胞,然后通过阻滞细胞周期G_0/G_1期,降低线粒体膜电位,线粒体去极化,最终诱导细胞凋亡。
Photodynamic therapy (PDT) has shown great potential in anticancer therapy as a rapidly evolving traditional alternative. In order to enhance the targeting and enhance the photocatalytic killing efficiency, a novel photosensitizer Fe 3 O 4 -Ti O 2 magnetic nanoparticles was designed in this study. Under different external magnetic fields, we investigated the killing effect on hepatocarcinoma cells under the excitation of visible light and UV light. At the same time, the effect of nano-Fe 3 O 4 -Ti O 2 on apoptosis rate, cell cycle and mitochondrial membrane potential of hepatoma cells was detected by flow cytometry. The anti-cancer mechanism was explored according to the mode of action of nano-Fe 3 O 4 -Ti O 2 and hepatoma cells. The results showed that the visible light excited nano-Fe 3 O 4 -Ti O 2 can kill the cancer cells, and the killing efficiency was not significantly different from that under ultraviolet light excitation. In addition, Fe 3 O 4 -Ti O 2 has higher cell uptake rate than Ti O 2, making it more selective and photocatalytic. The mechanism is that photocatalytic production of reactive oxygen species (ROS) in Fe 3 O 4 -Ti O 2 nanoparticles inhibits the growth of cancer cells, and then through the arrest of cell cycle G 0 / G 1, the mitochondrial membrane potential and mitochondrial depolarization are induced.