论文部分内容阅读
目的探讨革皮氏海参皂苷(saponin of Pearsonothuria graeffei,PGS)对骨髓抑制模型小鼠造血调控因子的影响。方法雄性ICR小鼠随机分为正常对照组、模型对照组和PGS低、高剂量组。剂量组小鼠灌胃不同浓度PGS(10和30 mg/kg),正常和模型对照组灌胃生理盐水,连续16d。除正常对照组外,其余小鼠均于首次PGS后第7d,连续3d腹腔注射环磷酰胺(100 mg/kg),建立骨髓抑制小鼠模型。于末次给药后,分别制备小鼠肺条件培养液(LCM)、脾条件培养液(SCM)和腹腔巨噬细胞条件培养液(PMΦCM),采用MTT法检测其对正常大鼠骨髓细胞增殖的影响;采用单层半固体培养法检测对粒单系祖细胞(CFU-GM)、红系祖细胞(CFU-E)和巨核系祖细胞(CFU-Meg)集落形成的影响;采用RT-PCR法检测粒/单核细胞集落刺激因子(GM-CSF)、促红细胞生成素受体(EPOR)和血小板生成素(TPO)mRNA的表达。结果经PGS体内诱导所制备的LCM、SCM、PMΦCM条件培养液能显著促进正常小鼠骨髓细胞增殖,增加CFU-GM、CFU-E和CFU-Meg祖细胞的集落形成,上调骨髓细胞GM-CSF、EPOR和TPO mRNA的表达量。结论 PGS通过诱导造血细胞因子的分泌促进骨髓粒单系、红系与巨核系造血的恢复。[营养学报,2012,34(6):599-604]
Objective To investigate the effect of saponin of Pearsonothuria graeffei (PGS) on the hematopoietic regulation factors of myelosuppression model mice. Methods Male ICR mice were randomly divided into normal control group, model control group and PGS low and high dose groups. The mice in the dose group were given gavage with different concentrations of PGS (10 and 30 mg / kg), and the normal and model control groups were given normal saline for 16 days. Except for the normal control group, the other mice were injected intraperitoneally with cyclophosphamide (100 mg / kg) on the 7th day after the first PGS to establish a myelosuppression mouse model. After the last administration, mouse lung conditioned medium (LCM), splenic conditioned medium (SCM) and peritoneal macrophage conditioned medium (PMΦCM) were prepared respectively and the proliferation of normal rat bone marrow cells (CFU-GM), erythroid progenitor cells (CFU-E) and megakaryocyte progenitor cells (CFU-Meg) were detected by single-layer semi-solid culture method. Method to detect the expression of granulocyte / monocyte colony stimulating factor (GM-CSF), erythropoietin receptor (EPOR) and thrombopoietin (TPO) mRNA. Results LCM, SCM and PMΦCM conditioned medium prepared by in vivo induction of PGS could significantly promote the proliferation of normal mouse bone marrow cells, increase the colony formation of CFU-GM, CFU-E and CFU-Meg progenitor cells and upregulate the expression of GM-CSF , EPOR and TPO mRNA expression levels. Conclusion PGS can promote the hematopoietic recovery of bone marrow mononuclear, erythroid and megakaryocyte by inducing the secretion of hematopoietic cytokines. [Journal of Nutrition, 2012,34 (6): 599-604]