目的：观察发育期（孕期和哺乳期）锌缺乏小鼠子代（成年）学习能力、海马长时程增强（ＬＴＰ）、脑组织金属硫蛋白（ＭＴ）及其ｍ ＲＮＡ的表达情况，初步探讨发育期锌缺乏影响脑功能及发育的机制。方法：将８０ 只刚怀孕ＩＣＲ雌鼠随机分为５组：严重缺锌、轻度缺锌、适锌、高锌对喂及高锌组，饲料含锌量分别为１，５，３０，１００ 及１００ ｍ ｇ／ｋｇ。各组小鼠在孕期和哺乳期均饲喂实验饲料，仔鼠断奶（出生第２０ 天）后改饲喂普通饲料。７０ ｄ 龄后，采用条件性回避反应模型测试仔鼠学习能力，然后在其海马脑片上进行诱导ＬＴＰ的实验；采用Ｗｅｓｔｅｒｎ ｂｌｏｔ和Ｎｏｒｔｈｅｒｎ ｂｌｏｔ技术检测其脑组织中ＭＴ蛋白及ＭＴ－Ⅰｍ ＲＮＡ 表达情况。结果：轻度缺锌组仔鼠达到学会标准所需次数明显多于高锌组（Ｐ＜ ０．００５），而其脑片上诱导的ＬＴＰ却显著低于高锌组（Ｐ＜０．００５）；脑组织中ＭＴ蛋白及ＭＴ－Ⅰｍ ＲＮＡ 表达量的顺序为严重缺锌组＜ 轻度缺锌组＜ 适锌组＜ 高锌对喂组＜ 高锌组。结论：发育期锌缺乏不但可造成学习能力下降、海马突触可塑性受损，同时还可使脑组织中ＭＴ蛋白及其ｍ ＲＮＡ 表达降低；ＭＴ蛋白转录水平受阻可能是锌缺乏引发脑功能损伤的重要分子 OBJECTIVE: To observe the offspring (adult) learning ability, hippocampal long-term potentiation (LTP), brain tissue metallothionein (MT) and m RNA expression during developmental period Mechanism of zinc deficiency during developmental period affecting brain function and development. Methods: Eighty pregnant women with just pregnant ICR were randomly divided into five groups: severe zinc deficiency, mild zinc deficiency, suitable zinc, high zinc on the feed and high zinc group, feed zinc content were 1,5,30,100 And 100 m g / kg. Mice in each group during pregnancy and lactation were fed experimental diets, offspring of weanling (20 days after birth) to change the general feed. After 70 days of age, the conditioned learning-avoidance model was used to test the learning ability of the offspring, and then LTP experiments were performed on their hippocampal slices. Western blot and Northern blot were used to detect the expression of MT protein and MT-ⅠmRNA . Results: The number of times needed to reach the standard of learning was significantly higher in mild zinc deficiency group than that in high zinc group (P <0.005), while the LTP in brain slices was significantly lower than that in high zinc group (P <0.005) ; The order of MT protein and MT-ⅠmRNA expression in brain tissue was severe zinc deficiency group