【目的】激发子可以诱导寄主植物的系统获得抗病性,具有有效性、持久性和广谱性的特点。研究旨在明确新型激发子寡糖·链蛋白(oligosaccharins·plant activator protein)对小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV)的免疫诱抗作用,为该激发子的研究和大面积推广应用提供技术支撑。【方法】选用小麦黄花叶病感病品种‘矮抗58’,在室内将消毒的小麦种子播种于自感病田带回的病土中,在(27±2)℃下培养。5叶期叶面喷施稀释1 000倍的6%寡糖·链蛋白。喷施7 d后,将麦苗置于(12±1)℃培养箱中接种培养。30 d后取出麦苗,分别测量小麦株高和叶片的叶绿素含量,计算病情指数和防治效果。在田间,同品种小麦种植于小麦黄花叶病常发地块,小麦返青后每周喷施1次6%寡糖·链蛋白,连续喷施3次。每周测量小麦株高和叶绿素含量,计算病情指数和防治效果。并于调查期间,每小区取20片植株最上部第一片完全展开的叶片,通过q PCR检测小麦植株内WYMV-CP基因拷贝数。在小麦收获时测定千粒重和穗粒数,测算产量。【结果】低温培养30 d后,经寡糖·链蛋白喷施处理的小麦较对照组的株高没有显著差异,但叶绿素含量则明显高于对照组(P<0.05);同时处理组的病情指数较对照组明显降低,防治效果达到63.32%。田间经寡糖·链蛋白处理后,小麦株高和叶绿素含量较对照没有显著差异。小麦返青期,喷施1周后病情指数与对照没有显著变化;而2周后病情指数显著降低,防治效果可达46.67%。小麦收获时调查发现,经寡糖·链蛋白处理后小麦穗粒数显著高于对照组(P<0.05),小麦产量明显升高(P<0.05)。病株内WYMY-CP基因拷贝数在喷施1周后抑制率达到69.30%,2周后达到85.50%,3周后最高达到99.20%。【结论】寡糖·链蛋白可诱导小麦植株对小麦黄花叶病毒的抗性,显著降低小麦植株内WYMV-CP基因拷贝数;在田间可以减轻小麦黄花叶病的危害,减少产量损失。 【Objective】 The elicitor can induce the host plant system to acquire the disease resistance, which has the characteristics of validity, persistence and broad spectrum. The aim of this study was to elucidate the immune-inducing effect of a new type of oligosaccharins plant activator protein on wheat yellow mosaic virus (WYMV), and to provide a theoretical basis for the research and popularization and application of the novel elicitor Technical Support. 【Method】 Wheat yellow mosaic virus susceptible cultivar ’Diankang 58’ was used in the experiment. The sterilized wheat seeds were sown indoors in the soil brought back from the susceptible field and cultured at (27 ± 2) ℃. 5 leaf stage foliar spray 1 000 times diluted 6% oligosaccharides · chain protein. After spraying for 7 days, the wheat seedlings were placed in a (12 ± 1) ℃ incubator and inoculated. After 30 days, the wheat seedlings were taken out, the wheat plant height and leaf chlorophyll content were measured, and the disease index and control effect were calculated. In the field, the same variety of wheat planted in wheat yellow mosaic leaf plots, wheat greening once weekly spraying 6% oligosaccharides · chain protein, spraying three times in a row. Wheat plant height and chlorophyll content were measured weekly to calculate disease index and control effect. During the investigation period, 20 plants in each plot were taken from the top of the first fully expanded leaf and the copy number of WYMV-CP gene in wheat plants was detected by q PCR. The grain weight and number of grains per spike were measured at the time of wheat harvest and the yield was measured. 【Result】 The results showed that the plant height of wheat treated with oligosaccharides and streptokinase was no significant difference but the content of chlorophyll was significantly higher than that of the control group (P <0.05) Index was significantly lower than the control group, the control effect of 63.32%. There was no significant difference in plant height and chlorophyll content between the two groups when treated with oligosaccharides and chain proteins. The wheat rejuvenation period showed no significant change in disease index and control after 1 week of spraying, while the disease index decreased significantly after 2 weeks, with the control effect reaching 46.67%. At the time of wheat harvesting, it was found that the grains per spike of wheat treated with oligosaccharides and chain proteins were significantly higher than that of the control (P <0.05) and the yield of wheat was significantly increased (P <0.05). The copy number of WYMY-CP gene in diseased plants was 69.30% after 1 week of spraying, 85.50% after 2 weeks, and up to 99.20% after 3 weeks. 【Conclusion】 Oligosaccharomycins can induce the resistance of wheat plants to wheat yellow mosaic virus and significantly reduce the copy number of WYMV-CP gene in wheat plants. It can reduce the damage of wheat yellow mosaic virus and reduce the yield loss in the field.