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目的 :研究Fas相关死亡结构域蛋白 (Fas associateddeathdomain ,FADD )在非小细胞肺癌 (non smallcelllungcan cer ,NSCLC )中的表达 ,并探讨其与NSCLC中癌细胞凋亡的关系。方法 :免疫组化 (IHC)SP法检测 62例NSCLC及13例癌旁肺组织中FADD蛋白表达 ,原位杂交法 (ISH)检测其中 3 0例肺癌组织FADDmRNA表达水平 ,TUNEL法检测NSCLC中癌细胞凋亡。结果 :NSCLC中FADD蛋白阳性表达率为 80 6%( 5 0 /62 ) ,低于癌旁肺组织 10 0 % ( 13 /13 ) ,但两者差异无统计学意义 ,P >0 0 5。癌组织中FADD表达多呈中度或强阳性 ,癌旁组织FADD多呈弱阳性 ,癌组织FADD表达程度高于非癌肺组织 ,χ2 =7 845 ,P <0 0 5。NSCLC中FADD表达程度与肿瘤的分化程度有关 ,低分化的肿瘤FADD表达减少 ,rs=0 411,P <0 0 1,但与患者性别、年龄、肿瘤分期及是否转移无关。原位杂交结果显示 ,FADDmRNA阳性率为 80 % ( 2 4/3 0 ) ,与相应组织FADD蛋白阳性率为 83 3 % ( 2 5 /3 0 ) ,两者差异无统计学意义 ,P >0 0 5。所有标本均可检测到癌细胞的凋亡 ,FADD表达水平与癌细胞凋亡程度之间呈显著正相关 ,rs=0 5 99,P <0 0 0 1。结论 :FADD表达异常在NSCLC的发生发展中可能起着重要作用 ,FADD表达水平与NSCLC细胞凋亡密切相关
Objective: To investigate the expression of Fas-associated death domain protein (FADD) in non-small cell lung cancer (NSCLC) and to explore its relationship with the apoptosis of NSCLC. Methods: Immunohistochemical (IHC) SP method was used to detect the expression of FADD protein in 62 cases of NSCLC and 13 cases of adjacent non-cancerous lung tissues. In situ hybridization (ISH) was used to detect FADD mRNA expression in 30 lung cancer tissues and TUNEL method to detect the expression of FADD protein in NSCLC Apoptosis. Results: The positive expression rate of FADD protein in NSCLC was 80 6% (50/62), which was lower than that in adjacent lung tissue (10 0%, 13/13). However, there was no significant difference between them (P> 0.05). The expression of FADD in cancerous tissue was mostly moderate or strong positive. The expression of FADD in paracancerous tissue was mostly weakly positive, while the expression of FADD in cancerous tissue was higher than that in non-cancerous lung tissue (χ2 = 7 845, P <0.05). The expression of FADD in NSCLC was correlated with the degree of tumor differentiation. The expression of FADD in poorly differentiated tumors was decreased, rs = 0 411, P 0 01, but not with gender, age, tumor stage and metastasis. In situ hybridization results showed that the positive rate of FADD mRNA was 80% (2 4/3 0) and the positive rate of FADD protein was 83 3% (25/30), with no significant difference (P> 0) 0 5. The apoptosis of cancer cells was detected in all the specimens. There was a significant positive correlation between the expression level of FADD and the degree of cancer cell apoptosis (rs = 0 5 99, P 0 001). Conclusion: The abnormal expression of FADD may play an important role in the occurrence and development of NSCLC. The expression of FADD is closely related to the apoptosis of NSCLC