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早在1972年,Hodeman等人在著名的厌氧菌实验室手册中,应用气相色谱法分析细菌代谢产物中的挥发性脂肪酸(VFA)和非挥发性脂肪酸(NVFA)作为厌氧菌分类的依据。目前,国外许多临床实验室已陆续将气相色谱分析作为鉴定厌氧菌的附加生化试验或常规检验项目之一,并试图用气相色谱法直接分析脓汁、感染组织浆液或脑脊液等临床标本,以进行厌氧性感染的快速推测性诊断。在此,仅对气相色谱技术在临床厌氧菌感染诊断中的应用作一简单介绍。
As early as 1972, Hodeman et al. Used gas chromatography to analyze the volatile fatty acids (VFA) and non-volatile fatty acids (NVFA) in bacterial metabolites as the basis for the classification of anaerobic bacteria in the well-known anaerobic laboratory manual . At present, many clinical laboratories abroad have successively adopted gas chromatography as one of the additional biochemical tests or routine tests to identify anaerobes. Attempts to use gas chromatography to directly analyze pus and infect clinical specimens such as tissue serous fluid or cerebrospinal fluid, Anaerobic infections for rapid speculative diagnosis. Here, only the gas chromatography in clinical diagnosis of anaerobic infections in the application made a brief introduction.