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目的探讨趋化因子受体CXCR4在胃癌和腹腔内转移灶中的表达及其意义。方法免疫组化法检测胃癌原发灶、淋巴转移灶、腹膜转移灶以及正常胃黏膜组织中的CXCR4表达情况。RT-PCR半定量检测胃癌原发灶、淋巴转移灶、腹膜转移灶以及正常胃黏膜组织中CXCR4 mRNA表达水平。结果免疫组化结果显示CXCR4在胃癌原发灶中的表达与肿瘤的分期、浸润深度及淋巴结转移有关(P<0.05)。RT-PCR结果显示早期胃癌中,进展期胃癌组织、淋巴结转移灶和腹膜转移灶中的CX-CR4 mRNA表达呈依次增强,各分组间CXCR4的表达差异有显著性(P<0.05)。结论CXCR4在原发灶中的表达与胃癌分期、肿瘤的浸润深度及淋巴结转移有关。表达CXCR4高的胃癌细胞更具侵袭性
Objective To investigate the expression of chemokine receptor CXCR4 in gastric cancer and intraperitoneal metastasis and its significance. Methods The expression of CXCR4 in primary gastric cancer, lymphatic metastasis, peritoneal metastasis and normal gastric mucosa was detected by immunohistochemistry. The mRNA expression of CXCR4 in primary gastric cancer, lymphatic metastasis, peritoneal metastasis and normal gastric mucosa tissues was semi-quantitatively detected by RT-PCR. Results The results of immunohistochemistry showed that the expression of CXCR4 in primary gastric cancer was related to tumor stage, depth of invasion and lymph node metastasis (P <0.05). The results of RT-PCR showed that the expression of CXCR4 mRNA in advanced gastric cancer tissues, lymph node metastasis and peritoneal metastasis was enhanced in turn, and the expression of CXCR4 in each group was significantly different (P <0.05). Conclusion The expression of CXCR4 in primary tumor is related to the staging of gastric cancer, the depth of tumor invasion and lymph node metastasis. Gastric cancer cells that express CXCR4 are more aggressive