目的利用HPLC-DAD法建立苦碟子中木犀草素7-O-β-D-葡萄糖苷(LGCOP)、木犀草素7-O-β-D-葡萄糖醛酸苷(LGCRP)和芹菜素7-O-β-D-葡萄糖醛酸苷(AGCRP)的测定方法。方法 Diamonsil C18色谱柱(200 mm×4.6 mm,5μm);流动相为(0.05%甲酸–水)–(0.05%甲酸–乙腈);二元梯度洗脱:检测波长340 nm;体积流量1.0 mL/min;柱温35℃;进样量10μL。结果在选定色谱条件下线性关系良好(r≥0.999 9)。平均回收率分别为100.5%、100.1%、100.8%,RSD值分别为0.4%、0.3%、0.7%。结论该分析方法能简便、快速地测定苦碟子中黄酮类成分,可为评价不同产地、药用部位及采收期的药材提供科学依据。 OBJECTIVE To establish a HPLC method for the determination of luteolin 7-O-β-D-glucoside (LGCOP), luteolin 7-O-β-D-glucuronide (LGCRP) and apigenin 7- Method for the determination of O-β-D-glucuronide (AGCRP). Methods Diamonsil C18 column (200 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of 0.05% formic acid and water (0.05% formic acid and acetonitrile). The binary gradient elution was performed at 340 nm. min; column temperature 35 ℃; injection volume 10μL. The results were linear under the selected chromatographic conditions (r ≥ 0.9999). The average recoveries were 100.5%, 100.1% and 100.8%, respectively. The RSDs were 0.4%, 0.3% and 0.7% respectively. Conclusion The method can be used to determine flavonoids easily and rapidly in Kudiezi. It can provide a scientific basis for the evaluation of medicinal materials in different producing areas, medicinal parts and harvesting period.