在诊断布氏菌病时,用抗体免疫反应试剂显示抗原和检验抗体的方法,已经有了较长的历史了。虽然观点有不同,但无可争议的是由于布氏菌和其它一些菌种,如 Yersiniaenterocolifica 09、Vibrio cholerae、N.E-scherichia coli 0157:H7类具有共同的抗原决定簇,所以用多克隆血清制备抗体免疫反应试剂能产生假阳性结果。由于杂交技术的 In the diagnosis of brucellosis, the use of antibody immunoreactive reagents to display antigens and antibody testing methods, has a long history. Although there is a difference in opinion, it is indisputable that polyclonal sera are used in the production of antibodies due to the common antigenic determinants of Brucella and some other species, such as Yersinia enterocolifica 09, Vibrio cholerae, and NE-scherichia coli 0157: H7 Immunoreactive reagents can produce false-positive results. Due to the hybrid technology