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在妊娠期某些血液成分的浓度,特别是蛋白和激素浓度增加至某一水平能抑制淋巴细胞在体外的增殖。据此观察提出在妊娠体内有一种免疫抑制作用。Morton等最早阐述早孕因子(EPF)是妊娠的最早信号之一,在受精的24-48h内即可测出。EPF有免疫抑制作用,因此在孕早期与抑制母亲免疫应答有关。胎儿流产与EPF失去活性有关的观察支持这一假设。为研究EPF对调节淋巴细胞活性的影响,作者采取了一种新的测定方法。对20名健康志愿者外周血用密度梯度离心,从管内界面收集外周血单核细胞(PBMC)经洗涤并混悬于无血清RPMI1640
The concentration of certain blood components during pregnancy, in particular the increase in protein and hormone concentrations, inhibits lymphocyte proliferation in vitro. According to this observation made in pregnancy has an immunosuppressive effect. One of the earliest signs of pregnancy, Morton et al. First described the early pregnancy factor (EPF), measured within 24-48 hours of fertilization. EPF has immunosuppressive effects, and therefore in the first trimester and suppression of the mother’s immune response. Observation of fetal abortion associated with loss of activity of EPF supports this hypothesis. In order to study the effect of EPF on the regulation of lymphocyte activity, the authors adopted a new assay. Peripheral blood from 20 healthy volunteers was subjected to density gradient centrifugation and peripheral blood mononuclear cells (PBMCs) collected from the in-tube interface were washed and suspended in serum-free RPMI1640