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利用常规杂交瘤技术制备了２株抗人绒毛膜促性腺激素（ｈＣＧ）的杂交瘤细胞。ＥＬＩＳＡ结果表明，单克隆抗体ＩＤ５和４Ｄ５均与人ｈＣＧ有特异性反应，腹水效价分别为３．２×１０－５、６．４×１０－５，均属于ＩｇＧ１亚类。与人促卵泡激素（ｈＦＳＨ）和人促黄体激素（ｈＬＨ）的交叉反应呈阴性。免疫转印结果显示能识别相对分子质量为２４×１０３ｕ的ｈＣＧ－β链条带。亲和常数分别为１．０１×１０７Ｌ／ｍｏｌ、４．４３×１０７Ｌ／ｍｏｌ。
Two anti-human chorionic gonadotropin (hCG) hybridoma cells were prepared by conventional hybridoma technique. The results of ELISA showed that the monoclonal antibodies ID5 and 4D5 both reacted specifically with human hCG. The ascites titer was 3.2 × 10-5 and 6.4 × 10-5, respectively, belonging to IgG1 subclass. Cross-reactivity with human follicle-stimulating hormone (hFSH) and human luteinizing hormone (hLH) was negative. Immunoblotting showed that the hCG-β chain with a molecular weight of 24 × 103u could be identified. The affinity constants were 1.01 × 107 L / mol and 4.43 × 107 L / mol, respectively.