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目的:探讨红细胞生成素诱导的肝细胞受体EphB4及其配体EphrinB2mRNA在子宫内膜癌组织中的表达及意义。方法:采用逆转录PCR(RT-PCR)法检测33例子宫内膜癌和10例正常子宫内膜组织中EphB4、EphrinB2mRNA的表达率及相对含量,并分析二者与子宫内膜癌临床病理特征的关系。结果:①EphB4mRNA在子宫内膜癌组的表达率和相对含量均高于正常子宫内膜组(P<0.05,P<0.05)。EphrinB2mRNA在子宫内膜癌组的表达率和相对含量也均高于正常子宫内膜组(P<0.05,P<0.01)。②EphB4、EphrinB2mRNA的表达与子宫内膜癌手术病理分期、组织学分级有密切关系(P<0.05)。二者表达与子宫内膜癌不同组织学类型、肌层浸润程度以及有无淋巴结转移无关(P>0.05)。结论:EphB4和EphrinB2的异常表达与子宫内膜癌发生、进展以及恶性程度有关,二者表达趋势呈现一致性,检测EphB4/EphrinB2有助于判断子宫内膜癌预后。
Objective: To investigate the expression of EphB4 and its ligand EphrinB2 mRNA in endometrial carcinoma induced by erythropoietin and its significance. Methods: The expressions of EphB4 and EphrinB2 mRNA in 33 cases of endometrial carcinoma and 10 cases of normal endometrium were detected by reverse transcription polymerase chain reaction (RT-PCR), and their clinicopathological characteristics Relationship. Results: ① The expression and relative content of EphB4 mRNA in endometrial carcinoma were higher than those in normal endometrium (P <0.05, P <0.05). The expression and relative content of EphrinB2 mRNA in endometrial carcinoma group were also higher than those in normal endometrium group (P <0.05, P <0.01). ② The expression of EphB4 and EphrinB2 mRNA was closely related to the pathological stage and histological grade of endometrial carcinoma (P <0.05). Both expression and endometrial carcinoma histological types, myometrial invasion and lymph node metastasis had no correlation (P> 0.05). Conclusion: The abnormal expression of EphB4 and EphrinB2 is related to the occurrence, progression and malignancy of endometrial carcinoma. The expression of EphB4 / EphrinB2 is consistent with the prognosis of endometrial carcinoma.