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阿米卡星本身在激发波长295nm,发射波长367nm处有微弱荧光。它在NaNO2存在下,氨基亚硝化为硝基,然后在弱酸条件下水解为还原糖后与乙二胺在磷酸盐缓冲液中反应会产生强荧光物质。据此建立了阿米卡星含量测定的新方法。阿米卡星在2.0×10-5～5.0×10-6 mol/L的范围内与其产物荧光强度呈现良好的线性关系,相关系数r=09952,检出限为4.2×10-7mol/L。该方法用于市售硫酸阿米卡星注射液含量的测定。
Amikacin itself has a weak fluorescence at an excitation wavelength of 295 nm and an emission wavelength of 367 nm. In the presence of NaNO2, the amino group is nitrosated to a nitro group, which is hydrolyzed to the reducing sugar under mild acid conditions and reacted with ethylenediamine in phosphate buffer to produce a strong fluorescent substance. Based on this, a new method for the determination of amikacin was established. Amikacin had a good linear relationship with the fluorescence intensity of its product in the range of 2.0 × 10-5 ~ 5.0 × 10-6 mol / L with a correlation coefficient of r = 09952 and a detection limit of 4.2 × 10-7mol / L. This method is used for the determination of amikacin sulfate injection.