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分别用不同温度、湿度、pH、光照对柑桔炭疽菌的不同分离系作单因子和复因子试验,结果表明:次生分生孢子形成的最适温度为23～27℃,最适pH值为6～7,在相对湿度为100%时产孢率最高,需要一定的营养。光对次生分生孢子形成的作用受温度影响,当温度在25℃以上产孢率最高的是黑光与黑暗交替和黑光的处理;温度在25℃以下时产孢率最高的是连续24小时的荧光处理。次生分生孢子形成必需要光。复因子试验的结果表明主效因素为温度,产孢率最高的最优组合为全黑暗—015—查彼培养液—pH8—水—25～29℃及先昼后夜—011—1%蛋白胨液—pH6—水—20～23℃。
Different temperature, humidity, pH, light were used to study the single and multiple factors of citrus anthrax isolates. The results showed that the optimal temperature for secondary conidia formation was 23 ~ 27 ℃, and the optimum pH value 6 to 7, the highest in the relative humidity of 100% when the sporulation rate, the need for some nutrition. The effect of light on the formation of secondary conidia is affected by temperature. When the temperature is above 25 ℃, the highest spore germination rate is the alternation of black light and darkness and the treatment of black light. When the temperature is below 25 ℃, the sporulation rate is the highest for 24 hours Fluorescence treatment. Secondary conidia formation requires light. Complex factor test results show that the main factors for the temperature, the highest sporulation rate of the best combinations of all dark -015-Cha Peter culture - pH8-water -25 ~ 29 ℃ and day-night night after -011-1% peptone Liquid-pH6-water -20 ~ 23 ℃.