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目的 :建立痫速停胶囊的含量测定方法。方法 :采用高效液相色谱法 ,HypersilBDSC18柱 (2 0 0mm× 4 .6mm ,5μm) ;流动相为乙腈 水 (40∶6 0 ) ;流速为 1ml·min-1;检测波长为 2 5 4nm。结果 :苯妥英钠和苯巴比妥分别在 15 0 .1～ 2 2 5 1.2μg·ml-1和 4 8.2～ 72 3μg·ml-1范围内线性关系良好 ;平均回收率分别为 10 0 .0 %及 10 0 .5 % (RSD 0 .8% ,0 .6 % )。结论 :本法简便、快速、准确。
Objective: To establish a method for determination of content of epilepsy capsules. METHODS: High performance liquid chromatography (HPLC) was performed on a Hypersil BDSC18 column (200 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and water (40:60). The flow rate was 1 ml · min-1 and the detection wavelength was 254 nm. RESULTS: Phenytoin sodium and phenobarbital had a good linear relationship in the range of 15 0 .1 ~ 2 2 5 1.2μg · ml-1 and 4 8.2 ~ 72 3μg · ml-1 respectively. The average recoveries were 10 0 .0 % And 100.5% (RSD 0.8%, 0.6%). Conclusion: This method is simple, fast and accurate.