近年来推广小量持续静脉滴注胰岛素疗法,但出现了容器对胰岛素吸附作用较大的弊病。作者应用HPLC测定容器吸附胰岛素稀溶液的作用及其防止的方法。层析条件、样品溶液的配制及测定方法同前报。结果与讨论:1.玻璃容器与输液管道吸附胰岛素情况:(1)经硅烷化处理的疏水性大的玻璃容器(安瓿),胰岛素回收率约为30％;未经处理的回收率约为70％。实验结果表 In recent years to promote a small amount of continuous intravenous insulin therapy, but the container has a greater role in the absorption of insulin ills. The author uses HPLC to determine the effect of container adsorption of insulin dilute solution and its prevention method. Chromatographic conditions, the preparation of the sample solution and determination of methods with the previous report. RESULTS AND DISCUSSION 1. Insulin adsorption in glass containers and infusion lines: (1) The silanized hydrophobic large glass containers (ampoules) had an insulin recovery of about 30% and an untreated recovery of about 70 %. Table of experimental results