为解决现有各种基因枪在微弹制备方面存在的问题并减小冲击波影响,提出一种基因枪结构。将裹着基因疫苗的微弹吸附在不锈钢网上,并将不锈钢网放置在加速通道中加速微弹。在数值模拟的基础上,研制了实验装置,测试了装置的性能,并利用该装置将pEGFG质粒导入H e la细胞。实验结果表明:这种结构的基因枪在0.3M Pa的气压下就可以把微弹加速到300m/s以上,且微弹在靶面分布均匀;射入H e la细胞的pEGFG质粒也得到了表达,能满足基因疫苗接种的要求。 In order to solve the problems existing in preparation of micro-bombs and reduce the influence of shock waves, a gene gun structure was proposed. Will be wrapped in the gene vaccine micro-absorption on the stainless steel mesh, and stainless steel mesh placed in the acceleration channel to accelerate micro-shells. Based on the numerical simulation, an experimental device was developed to test the performance of the device, and the pEGFG plasmid was introduced into Hela cells by using the device. The experimental results show that the particle gun of this kind of structure can accelerate the micro-bomb to 300 m / s or more under the pressure of 0.3M Pa, and the micro-bomb distributes uniformly on the target surface. The pEGFG plasmid injected into H e la cells is also obtained Expression, to meet the requirements of genetic vaccination.