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目的 为阐明麝香保心丸改善心肌血流灌注的机理。方法 在高脂血症造成兔动脉壁一氧化氮代谢异常的动物模型上，采用定量ＲＴ－ ＰＣＲ 等技术，观察了该药的干预作用。结果 高脂饮食可造成动脉一氧化氮代谢的紊乱，麝香保心丸可部分逆转这种损害；与高脂血症对照组相比，它有提高动脉壁内皮型一氧化氮合酶（ｅＮＯＳ） 基因表达的趋势（０－１＞ Ｐ＞ ０－０５） 、显著增强动脉的ＮＯＳ 的活力（ Ｐ＜ ０－０５） 、提高血浆一氧化氮代谢产物（ＮＯＰ） 的水平（Ｐ＜０－０５） 。结论 麝香保心丸的抗心肌缺血作用可能与保护动脉壁一氧化氮系统有关。
Objective To elucidate the mechanism of Shexiang Baoxin Pill in improving myocardial perfusion. Methods The animal model of abnormal nitric oxide metabolism in rabbit arterial wall caused by hyperlipidemia was used quantitative RT-PCR to observe the intervention effect of this drug. RESULTS High-fat diet caused a disorder of the metabolism of nitric oxide in the arteries. This was partially reversed by Shexiang Baoxin Pill; it had enhanced endothelial nitric oxide synthase (eNOS) compared with the hyperlipidemia control group. Trends in gene expression (0-1>P>0-05), significant enhancement of arterial NOS activity (P<0-05), and increase in plasma nitric oxide metabolite (NOP) levels (P<0-05) . Conclusion The anti-myocardial ischemia effect of Shexiang Baoxin Pill may be related to the protection of the nitric oxide system in the arterial wall.