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目的:了解诱癌过程中大鼠免疫功能变化,观察大鼠IL-2、T淋巴细胞亚群动态变化及其相互关系。方法:应用二甲基奶油黄(DAB)诱发大鼠肝癌,在诱发大鼠肝癌的3个阶段分别取大鼠脾脏和外周血测定IL-2活性和T淋巴细胞亚群。结果:肝炎、肝硬变、肝癌期大鼠IL-2活性、Th降低有显著性,P<0.05或<0.01。肝癌期大鼠Ts显著增加,P<0.05。肝炎、肝硬变期IL-2活性与Th正相关,肝癌期大鼠IL-2活性与Ts负相关,与Th/Ts比值呈正相关。结论:诱发大鼠肝癌过程中,IL-2活性逐步降低,良性肝病期IL-2活性与Th下降有关,肝癌期IL-2活性与Ts增加和Th/Ts比值降低有关。肝炎期IL-2活性显著下降提示有早期肝癌发生的可能。
Objective: To understand the changes of immune function in rats during the process of carcinogenesis and observe the dynamic changes of IL-2 and T lymphocyte subsets in rats and their relationship. METHODS: Rat liver cancer was induced by dimethyl cream yellow (DAB). Rat spleen and peripheral blood were taken to determine IL-2 activity and T-lymphocyte subsets in the three stages of rat liver cancer. RESULTS: The activity of IL-2 and the decrease of Th were significantly different in rats with hepatitis, hepatic cirrhosis and hepatocellular carcinoma, P<0.05 or <0.01. Ts increased significantly in rats with hepatocellular carcinoma, P<0.05. The activity of IL-2 was positively correlated with Th in hepatitis and cirrhosis phases. The activity of IL-2 in rats with hepatocellular carcinoma was negatively correlated with Ts and positively correlated with Th/Ts ratio. Conclusion: The activity of IL-2 is gradually decreased during the process of hepatocarcinogenesis in rats, and the activity of IL-2 in benign liver disease stage is related to the decrease of Th. The activity of IL-2 in hepatocellular carcinoma is related to the increase of Ts and the decrease of Th/Ts ratio. A significant decrease in the activity of IL-2 during the period of hepatitis suggests the possibility of early liver cancer.