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人早幼粒白血病细胞株(HL-60)在体外分别与1,25二羟维生素D3(1,25(OH)_2D_3)、维甲酸、二甲基亚砜(DMSO)和TPA共同培养5天后,在上述各分化诱导剂的作用下可向单核-巨噬细胞或粒细胞分化。在其分化前后,用抗单核-巨噬细胞或粒细胞的各种单克隆抗体进行间接免疫荧光检查,以比较诱导分化的白血病细胞与处于相应分化阶段的正常细胞抗原性是否相同。结果表明,TPA和1,25(OH)_2D_3(10~(-8)M)可诱导单核-巨噬细胞分化。组化分析证明,ANAE中度或强阳性的细胞经诱导培养后由原来的8％升至73％。但是,经1.25(OH)_2D_3诱导的细胞形态类似单核细胞,可与单克隆抗体FMC17及FMC32有较强的结合
Human promyelocytic leukemia cell line HL-60 was cultured in vitro with 1,25-dihydroxyvitamin D3 (1,25 (OH) 2D3), retinoic acid, dimethylsulfoxide (DMSO) and TPA Can differentiate into monocytes-macrophages or granulocytes under the action of the above differentiation inducing agents. Before and after differentiation, indirect immunofluorescence was performed using various monoclonal antibodies against monocytes-macrophages or granulocytes to compare whether the differentiated leukemic cells are antigenic with normal cells at the corresponding differentiation stage. The results showed that TPA and 1,25 (OH) _2D_3 (10 ~ (-8) M) induced monocyte-macrophage differentiation. Histochemistry analysis showed that moderate or strong ANAE-positive cells increased from 8% to 73% after induction. However, the morphology of cells induced by 1.25 (OH) _2D_3 was similar to that of monocytes, which could be strongly associated with the monoclonal antibodies FMC17 and FMC32