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目的采用ITS序列对山姜属物种进行分子鉴别。方法通过PCR扩增测序或Genbank下载获取山姜属物种的ITS序列,采用DNAMAN软件对所有序列进行序列校正,使用Clustal X软件进行序列多重比对,运用MEGA 4.0软件计算遗传距离和构建系统发育树。结果山姜属24个物种43条序列比对后序列为509 bp,变异位点共122个。高良姜等19个物种的种间遗传距离大于其种内遗传距离,具有明显的条形码间距,可与其他物种区别;疏花山姜等5个物种种间遗传距离小于或等于种内距离,不能完全被鉴别开来。系统聚类树显示,大多数山姜属物种各自聚成一分支,单系性较好。结论 ITS序列对24个山姜属物种具有良好的鉴别能力,为该属物种鉴定研究提供了分子依据。
Objective To identify the species of the genus Ginger by ITS sequence. Methods The sequences of ITS were sequenced by PCR amplification or Genbank. DNAMAN software was used to sequence the sequences. Sequence alignment was performed by Clustal X software. The genetic distance was calculated by using MEGA 4.0 software and phylogenetic tree . Results The sequence of 43 sequences of 24 species of the genus Gingeria was 509 bp and the number of variation sites was 122. The genetic distance among 19 species such as galangal was greater than their intra-species genetic distance, with obvious bar-code spacing, which could be distinguished from other species. The genetic distance between 5 species of Grifola species was less than or equal to the intra-species distance and could not be completely Be identified. The phylogenetic tree shows that most of the species of the genus Ginger belong to one branch and the monophyleticity is better. CONCLUSION ITS sequence has good discriminating ability on 24 species of Sinapis, which provided the molecular basis for the identification of this species.